Antibody Expression with the TGEX Vector Series

A tool for rapid transient expression of antibodies.

P

hage display only produces phage clones. Between them and fully recombinant antibodies expressed in stable cell lines lies a grey zone where rapid access to free, recombinant molecules becomes a necessity. The TGEX™ transient expression vector series has been designed to bridge this gap and allows for the rapid expression of recombinant antibodies by transient transfection.

The TGEX™ Vector Backbone

The TGEX™-Zeo backbone features a CMV promoter, the adenovirus tripartite leader sequence (TPL) (Logan 1984, Mariati 2010), a powerful hybrid leader sequence, a convenient cloning site to insert the antibody sequence, followed by an IRES element and the Sh ble gene from Streptoalloteichus hindustanus conferring resistance to Zeocin and terminated by the woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) and the rabbit beta-globin polyadenylation signal (BGPA). Using widely available cell lines and large scale transfection technologies, yields of antibody between 20 mg/L and 200 mg/L, or more, are routinely achieved in the laboratory in a few days. Selection with Zeocin results in the creation of stable pools with, in the case of two plasmids, an equal distribution of the expressions, here illustrated with eGFP and DsRed, allowing the concomitant selection of the antibody heavy chain and the antibody light chain with a single antibiotic.

Large Collection of Isotypes Available

Adapted to Multiple Antibody Formats

Fab fragments, Fc fusions, scFv-Fc/VHH fusion, secreted proteins.

Control Vectors

Vector maps, sequences, full description and ordering…

The TGEX-SCblue vector is designed for the conversion of scFv-bearing phage clones into scFv-Fc fusions for antibody validation after biopanning.

References