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ALTHEA Gold™ Antibody Library Kit
Technical Description
The ALTHEA Gold™ Antibody Libraries are advanced semi-synthetic human antibody phage display libraries for therapeutic antibody discovery (1). The libraries are built on two framework combinations, VK3-20/VH3-23 and VK-4-1/VH3-23, to modulate the shape of the paratope and enable the recognition of a wide range of potential protein & peptide targets. Sequence diversity is concentrated specifically in the CDRs, at key antibody-antigen interaction sites that were identified through comprehensive analysis of known antibody-antigen structures, except for the CDR-H3, which are of human origin and derived from a large pool of donors. More information here.
Figure 1. Principle of construction of the ALTHEA Gold libraries.
The synthetic primary libraries in scFv format were exposed to a heat-shock and rescued by protien A to eliminate unfit cones and select for thermostable, developable antibodies (Fig. 1). A subsequent shuffling using H3J fragments derived from human donors resulted in a 100-fold increase in diversity and a 50% increase in thermostability. Each library contains more than 10 billions unique clones..
Applications
-
Antibody discovery.
-
Therapeutic antibodies.
For research use only; not intended for any animal or human therapeutic or diagnostic use.
Kit Components
The following reagents are included with the kit:
|
Product |
Composition |
Amount |
|---|---|---|
|
SL1 VH23-VL3-20 Phage |
10 OD 269 nm, ~ 2 x 1013 cfu/ml, supplied in TBS |
250 μl |
|
SL2 VH23-VL4-01 Phage |
10 OD 269 nm, ~ 2 x 1013 cfu/ml, supplied in TBS |
250 μl |
|
PHIS4 PRIMER |
5’-GCGGATAACAATTTGAATTCAAGGAGACAG, 1 nmol, 10 μM, 10 pmol/μl |
100 μl |
|
PSIR2 PRIMER |
5’-CGTTAGTAAATGAATTTTCTGTATGAGG, 1 nmol, 10 μM, 10 pmol/μl |
100 μl |
|
PSIR32 PRIMER |
5’-GCGTAACGATCTAAAGTTTTGTCG, 1 nmol, 10 μM, 10 pmol/μl |
100 μl |
|
TG1 |
Phage-Competent™ TG1 cells, 20 OD600/ml, >2 x 1010 cfu/ml |
0.5 ml |
|
SS320 |
Phage-Competent™ SS320 cells, 20 OD600/ml, >2 x 1010 cfu/ml |
0.5 ml |
Associated Products
The following products can be used during panning:
|
Product |
Composition |
Amount |
|---|---|---|
|
M13KO7 Helper Phage (concentrated) |
1 ml |
|
|
CM13 Helper Phage (concentrated) |
1 ml |
|
|
Phage-Competent™ TG1 cells, 20 OD600/ml, >2 x 1010 cfu/ml |
10 x 0.5 ml |
|
|
Phage-Competent™ SS320 cells, 20 OD600/ml, >2 x 1010 cfu/ml |
10 x 0.5 ml |
Quality Control & Certification of Analysis
Quality Control Assays
Specifications for each lot are reported in the certificate of analysis.
Certification
Product meet all specifications.
References
- Valadon P. & al. (2019). ALTHEA Gold Libraries™: antibody libraries for therapeutic antibody discovery. MAbs, 11(3):516-531.
Citations
-
Vidal-Calvo EE et al. (2024). Tumor-agnostic cancer therapy using antibodies targeting oncofetal chondroitin sulfate. Nat Commun 2024;15(1):7553.
-
Zhang Y. (2023). Evolution of phage display libraries for therapeutic antibody discover. MAbs 2023;15(1):2213793.
-
Guzmán-Bringas OU. et al. (2023). Discovery and Optimization of Neutralizing SARS-CoV-2 Antibodies Using ALTHEA Gold Plus Libraries™. Int J Mol Sci. 2023;24(5):4609.
-
Mendoza-Salazar I. et al. (2022). Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display Library. Antibodies (Basel). 2022;11(1):13.
-
Dao T. et al. (2022). A TCR mimic monoclonal antibody for the HPV-16 E7-epitope p11-19/HLA-A*02:01 complex. PLoS One. 2022;17:17(3):e0265534.
-
Dao T. et al. (2022). A TCR mimic monoclonal antibody reactive with the "public" phospho-neoantigen pIRS2/HLA-A*02:01 complex. JCI Insight. 2022;8;7(5):e151624.
-
Pedraza-Escalona M. et al. (2019). Isolation and characterization of high affinity and highly stable anti-Chikungunya virus antibodies using ALTHEA Gold Libraries™. BMC Infect Dis. 2021;21(1):112.
-
Almagro J. et al. (2019). Phage Display Libraries for Antibody Therapeutic Discovery and Development. Antibodies (Basel). 2019;23;8(3):44.
-
Valadon P. & al. (2019). ALTHEA Gold Libraries™: antibody libraries for therapeutic antibody discovery. MAbs 2019;11(3):516-531.