TGEX-FC-hG1-Zeo

TGEX-FC-hG1-Zeo

Cloning Vector for the Mammalian Expression of Human IgG1 Fc Fusion Molecules

Technical Description

TGEX™-FC-hG1-Zeo is designed for the expression of N-terminal fusions with the Fc region of human IgG1. Using transient transfection technologies yields of fusion molecules between 20 mg/L and 200 mg/L and more in serum-free conditions can be obtained in just a few days.

Applications

• Mammalian expression of N-terminal Fc fusion molecules by transient gene expression.

• Selection of stable transfectants using Zeocin™.

For research use only; not intended for any animal or human therapeutic or diagnostic use.

Specifications

General Characteristics:

Plasmid Size: 5519
Promoter: CMV + TPL
Leader Peptide: pelBK
Cloning Site: Double BsaI
Fusion Protein: human IgG1 Fc region
Selection: ampicillin (bacteria), zeocin™ (mammalian)
Replication: pMB1 high copy number

Physical Characteristics:

Concentration: 0.5 µg/µl.
Product Size: 10 µg.
Buffer: DNA Conservation Buffer (Tris/HCL 5 mM, EDTA 0.1 mM, pH 8.5, sterile).
Storage Temperature: -20°C.

Quality Control & Certification of Analysis

Product Sequence:

The entire plasmid sequence was confirmed by the Sanger method.

Certification:

Product meets all specifications.