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TGEX-AC-Zeo
Mammalian Expression Vector
Technical Description
The TGEX™-AC-Zeo is a mammalian expression vector designed for the secretion of recombinant proteins in the culture supernatant. As such, TGEX™-AC-Zeo is the precursor of all TGEX™ antibody vectors for the expression of antibody molecules. Using transient transfection technologies, many recombinant proteins yield between 10 mg/L and 200 mg/L and more in serum-free conditions in just a few days.
Applications
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Mammalian secretion of recombianat proteins by transient gene expression.
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Selection of stable transfectants using Zeocin™.
For research use only; not intended for any animal or human therapeutic or diagnostic use.
Specifications
General Characteristics:
Plasmid Size: 4838
Promoter: CMV + TPL
Leader Peptide: pelBK
Cloning Site: Double BsaI
Selection: ampicillin (bacteria), zeocin™ (mammalian)
Replication: pMB1 high copy number
Physical Characteristics:
Concentration: 0.5 µg/µl.
Product Size: 10 µg.
Buffer: DNA Conservation Buffer (Tris/HCL 5 mM, EDTA 0.1 mM, pH 8.5, sterile).
Storage Temperature: -20°C.
Quality Control & Certification of Analysis
Product Sequence:
The entire plasmid sequence was confirmed by the Sanger method.
Certification:
Product meets all specifications.
Citations
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Mendoza-Salazar I., Gómez-Castellano KM., González-González E., Gamboa-Suasnavart R., Rodríguez-Luna SD., Santiago-Casas G., Cortés-Paniagua MI., Pérez-Tapia SM., Almagro JC. (2022). Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display Library. Antibodies (Basel),10;11(1):13.
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Pedraza-Escalona M., Guzmán-Bringas O., Arrieta-Oliva HI., Gómez-Castellano K., Salinas-Trujano J., Torres-Flores J., Muñoz-Herrera JC., Camacho-Sandoval R., Contreras-Pineda P., Chacón-Salinas R., Pérez-Tapia SM, Almagro JC. (2021). Isolation and characterization of high affinity and highly stable anti-Chikungunya virus antibodies using ALTHEA Gold Libraries™. BMC Infect Dis.,30;21(1):1121.
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Mukherjee A., Stathos ME., Varner C., Arsiwala A., Frey S., Hu Y., Smalley DM., Schaffer DV., Kane RS. (2020). One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathway. Chem. Commun. (Camb) 56(25):3685-3688.