Anti-M13 g8p Antibody (HRP) - 500 µg

Anti-M13 g8p Antibody (HRP) - 500 µg

Available Quantities

AS003-50 - Anti-M13 g8p Antibody (HRP) - 50 µg
AS003-100 - Anti-M13 g8p Antibody (HRP) - 100 µg
AS003-500 - Anti-M13 g8p Antibody (HRP) - 500 µg

Technical Description

Anti-M13 g8p antibody clone A4B1 is a mouse monoclonal developed especially for phage ELISA. The antibody recognizes an epitope of the main coat phage protein pVIII exposed at the virion surface, resulting in a very strong signal amplification after binding along the phage filament. As low as 10^7-10^8 cfu/ml can be detected giving one of the highest sensitivity in the field (Fig. 1).

Figure 1. Phage ELISA. M13 phage were captured by a bound anti-M13 monoclonal antibody and detected with secondary anti-M13-HRP conjugates at 0.2 µg/mL.

Applications

• Phage ELISA with M13 filamentous phage, phage display. Recommended dilution 1/5000 (0.2 μg/mL).

For research use only; not intended for any animal or human therapeutic or diagnostic use.

Specifications

General Characteristics:

Source: Recombinant murine IgG2a/K
Immunogen: M13 phage
Specificity: Major coat protein g8p of M13 filamentous bacteriophage.
Clone: A4B1
Purification: Protein A affinity chromatography.
Purity: >95%

Physical Characteristics:

Concentration: 1.0 mg/ml.
Product Size: 500 µg.
Buffer: PBS plus ethylene glycol.

Stability:

Storage Temperature: -20°C.
Expiration Date: 1 year from delivery.

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