FAST-Licase - 20 rxns

FAST-Licase - 20 rxns

Technical Description

FAST-Licase™ enables the ultra-fast seamless cloning of two or more DNA fragments. This cloning method is based on the homologous recombination of short, 15 to 20 bp-long overlaps between fragments resulting in precise and unique cloning events. The licase™ complex comprises a series a DNA modifying enzymes that are sequentially activated to achieve an extremely high cloning efficiency in just a few seconds. Competent bacteria with medium transformation efficiency in the range 1 x 10⁸ transformants/µg are usually sufficient to complete cloning projects, thus saving both on time and expenses.

Learn nore on the Licase™ Cloning Method and its applications

Applications

• Assembly of 2 or more DNA fragments.

• Cloning of large DNA fragments.

• Mutagenesis.

• Insertion of short DNA sequences.

Features

• DNA assembly between 2 s and 2:30 min (water bath or thermo-cycler).

• Very high insertion rate.

• Incredible time saver.

• High-efficiency competent bacteria unneeded.

• No positive control required.

For research use only; not intended for any animal or human therapeutic or diagnostic use.

Specifications

Physical characteristics:

Licase™ Complex: 20 µl.
Buffer 5x: 200 µl.
Storage Temperature: -20°C.

Quality Control & Certification of Analysis

Certification

Product meet all specifications.