Antibody Reformatting

Any format, any isotype, any species.

Overview

We provide cloning & reformatting services for your antibodies: scFv, scFv-Fc fusions, Fab, Fab’2, full-length IgG and more. Switch variants and chimeric antibodies can be produced in varied isotypes and the species of your choice, including human IgM, IgA, IgG1, IgG3 and IgG4 with either kappa or lambda light chain, all murine, rat, rabbit and dog IgGs. Other species and isotypes are available on demand. More specialized constructions are welcome such as Fc or albumin fusions and minibodies.

Formats

  • scFv. Single chain Fv (scFv) with any linker. The scFv can be made from synthetic DNA, for example derived from a database accession number or the sequencing of your hybridoma cell line.
  • scFv-Fc Fusions. A convenient format to reproduce binding of the parent antibody with minimum loss of affinity, at best made using our scFv-Fc fusion platform.
  • Minibodies. scFv-CH3 fusions or minibodies use the CH3 domain for dimerization and have a small size, making them a good choice when tissue penetration and clearance are desired pharmacokinetic properties, e.g. for tumor imaging.
  • Fab fragments. The preferred format when monovalency is desired. Fab fragments can be rapidly expressed by mammalian transient transfection using our TGEX vector expression platform.
  • Full-Length IgG. Ideal for isotype switching and chimeric antibodies or simply making full-length recombinant antibodies from any variable domain sequences. Full-length IgG can be rapidly expressed by mammalian transient transfection using our TGEX vector expression platform.
  • Fc Mutations & Activity Modification. We can introduce the mutations of your choice in the Fc portion of the antibody molecule to modulate antibody function and clearance. Some variants, including aglycosylated human IgG1, IgG1 mutant with increased serum half-life, and IgG1 and IgG4 clones with modulated ADCC and CDC are available on our catalog.
  • Fc Fusions. A convenient way to reach a target, retain antibody function and achieve long half life.
  • Albumin Fusions. Often used to increase the half life of circulating polypeptides.

Project

Starting material

A hybridoma sequence, a phage clone, a scFv or a Fab sequence, a sequence from your repository or a GenBank accession number is required to start the project.

Cloning & expression

The light chain and the heavy chain will be transferred in one of our expression vectors optimized for high level expression in the desired format; custom vectors provided by the client can be accommodated as well. We offer customization with varied tags for assay detection, purification purposes or scaffolding by multimerization; tags for purification can be later removed by specific protease cleavage, depending on your purification schema.

Deliverables

  • Detailed project report.

  • Annotated sequences & sequencing traces.

  • Plasmid map of each construct.

  • Purified plasmid DNA for each construct.

Cost & Turnaround Time

Please ask for a custom quote. Expect rapid turnover; we combine cloning and expression on request.

Information Request

P

lease, request a specific quote by completing the online quotation form accessible by pressing the following button; you will be contacted back by the next business day.

Contact Us

For all other inquiries, contact us by email at info@abdesignlabs.com or by phone at 1-877-223-3104 (PST). We guarantee competitive pricing.

All our work is performed by highly experienced staff at our San Diego facility in California. All products and services are for research use only and are not intended for use in humans.

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