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New Phagemid Vectors from the pADL™-20 Series Mimics pCOMB3
Published November 24, 2013
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hagemid vectors pADL-20c, pADL-22c, and pADL-23c are now available in store. To get full advantage of the amber suppression for expression of soluble antibodies and direct detection without sub-cloning, we adjusted the level of expression to match the high expression vector pCOMB3, a popular phagemid known for its robust display and moderate toxicity. A transcription terminator has been added after gene III to prevent excessive production of beta-lactamase during induction period.
Custom Immunized Phage Display Antibody Libraries
Published April 29, 2013
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e now offer the design and building of antibody libraries from immunized animals as a service. You provide the antigen; we conduct the immunization, design and build the antibody library for you as a service. Phage display is a straightforward approach to isolate high affinity monoclonal antibodies from small, focused libraries build from immunized animals. These libraries are of moderate size, between 1 and 10 million clones and usually mirror the animal immune response; high affinity antibodies, similar to antibody generated by hybridoma technology, are isolated from them by straightforward selection techniques. We have extensive experience in building phage antibody libraries in varied formats and display types.
Custom Phage Display Peptide Libraries
Published April 11, 2013
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e now offer the construction of custom phage display peptide libraries as a service. With more than 20 years of experience in the field, we are offering our expertise in the construction of peptide libraries on phage as a service for your research and development efforts. Your library will be designed with introduction of diversity precisely controlled and parameters that determine selection carefully chosen and finally build using robust methods to achieve the target size with limited loss of diversity.
CM13 Interference-Resistant Helper Phage
Published March 26, 2013
CM13 Interference-Resistant Helper Phage is a new, well-characterized mutant of M13KO7 producing on average twice as many virions than M13KO7 with the same level of display. CM13 is well-tolerated by the bacterial host and is compatible all filamentous phage display systems. Our new formulation gives you enough helper to surperinfect up to 1,000 ml of bacterial culture.
pADL Phagemid Vector Series Update
Published October 30, 2012
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ew versions of the pADL phagemid vectors pADL-20b, pADL-22b, and pADL-23b are now available. The sequence of the Amp(R) gene sequence has been modified, allowing the cloning site to be opened by either SfiI or BglI restriction enzyme. This modification results in lower background during cloning and brings more flexibility for designing libraries. The pADL vector series is a collection of phagemid vectors for controlled display on the minor coat protein III which combines HIS tag for purification, epitope tag for detection and amber stop codon for conditional display. Expression of the fusion protein is tightly controlled, eliminating issues with toxic clones and library misrepresentation.