Antibody Expression with the TGEX Vector Series
A tool for rapid transient expression of antibodies.P
hage display only produces phage clones. Between them and fully recombinant antibodies expressed in stable cell lines lies a grey zone where rapid access to free, recombinant molecules without purification & detection headaches can become a challenge. The TGEX transient expression vector series has been designed to bridge this gap
The TGEX Vector Backbone
The TGEX™ backbone features a CMV promoter, the adenovirus tripartite leader sequence (TPL) (Logan 1984, Mariati 2010), a variable antibody domain leader sequence with its intron and convenient cloning sites to insert antibody variable regions. The constant regions are derived from the human IgG1 and kappa sequences. There is no selection marker and the overall small size of the vectors is optimized for transient transfection; expensive antibiotics to prepare large quantities of plasmid for transient transfections are not required. Using widely available cell lines and large scale transfection technologies (see manuals) yields of antibody between 10 mg/L and 100 mg/L in serum-free conditions are routinely achieved in the laboratory in just a few days.
Adapted to Multiple Antibody Formats
Full IgG, Fab fragments, Fc fusions, scFv-Fc fusion.
Vector maps, sequences, full description and ordering…
The TGEX-SCblue vector is designed for the conversion of scFv-bearing phage clones into scFv-Fc fusions for antibody validation after biopanning.
1. Logan, J., & Shenk, T. (1984). Adenovirus tripartite leader sequence enhances translation of mRNAs late after infection. Proceedings of the National Academy of Sciences of the United States of America, 81(12), 3655–9.
2. Mariati, Ho, S. C. L., Yap, M. G. S., & Yang, Y. (2010). Evaluating post-transcriptional regulatory elements for enhancing transient gene expression levels in CHO K1 and HEK293 cells. Protein Expression and Purification, 69(1), 9–15.
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