Custom Phage Display Antibody Libraries

Service Description

Phage display is a straightforward approach to isolate high affinity monoclonal antibodies from small, focused libraries build from immunized animals. These libraries have a typical size between 1 x 108 and 1 x 109 clones and mirror the animal immune response. High affinity antibodies, similar to antibody generated by hybridoma technology, are isolated from them by straightforward selection techniques. We have extensive experience in building phage antibody libraries in varied formats and display types. You provide the antigen; we conduct the immunization campaign, design and build the antibody library for you as a service.

  • Tailored immunization campaigns.

  • Library size between 108 and 109 or more.

  • Standard size is 4 x 108 in-frame antibody clones.

  • Choice of vector, tags, and helper phage (M13KO7, CM13, CM13d3).

  • Choice of format, Fab or scFv.

  • Specific vectors for improved Fab display.

  • Seamless Fab library construction using our universal phage display (pUP) system (Fig. 1).

Figure 1. Fab Library Cloning in pUP display system. The universal pUP vector, amplified VL and VH domain are ligated in a single cloning step to generate highly diverse Fab libraries with no bias toward light chains or heavy chains and large coverage of the antibody response (Nelson 2017).

Immunized Murine Antibody Libraries.

Phage display enables selective screens to isolate antibodies that are not accessible by classical hybridoma technology. We will immunize mice with your antigen; the spleen of the best responder will be harvested and used to amplify cDNA and build the library. We provide you with the bleeds to make your own testing.

Llama Fab & scFv Discovery

Llama are a great source of antibodies and are mostly used for the discovery of single domain VHH. Llamas and alpacas also have regular VH/VL antibodies that make great IgG1 responses with specificities often non-detected in the VHH IgG2/3 compartment. We have analyzed the llama light chain and heavy chain repertoire and we offer, as a service, the construction of immunized camelid Fab and scFv phage display libraries in addition to single domain phage display libraries.

Antibody Format & Display Type, Species

You can choose from any of our phagemid vectors to build the library. We can build scFv and Fab libraries; specific format and tags will be decided together. We offer antibody discovery services routinely for the following species: human, mice, rat, llama, dogs. Please, contact us directly for other species.

Cost & Turnaround time

P

lease, request a specific quote by completing the online quotation form accessible by pressing this button; you will be contacted back by the next business day.

Request A Quote

For all other inquiries, contact us by email at info@abdesignlabs.com or by phone at 1-877-223-3104 (PST). We guarantee competitive pricing.

All our work is performed by highly experienced staff at our San Diego facility in California. We use highly efficient cloning techniques to ensure timely delivery and have very high standards for validation. All products and services are for research use only and not intended for use in humans.

References

  1. Nelson, R.S., & Valadon, P. (2017). A universal phage display system for the seamless construction of Fab libraries. J Immunol Methods, 450, 41-49.

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