LOCUS       pADL-10b                4990 bp    DNA     circular
DEFINITION  Phage display vector pADL-10b, complete sequence.
ACCESSION   
VERSION     .0
KEYWORDS    .
COMMENT     
FEATURES             Location/Qualifiers
     CDS             126..986
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
     rep_origin      1141..1760
                     /label=pMB1
                     /gene="pBR322 origin"
     CDS             1888..2970
                     /codon_start=1
                     /product="LacI transcriptional repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ*"
                     /gene="LacI"
                     /label=LacI
                     /note="Start with ATG in BL21 genome"
     misc_binding    2973..2978
                     /db_xref="REBASE:KpnI"
     terminator      2981..3017
                     /label=tHP_terminator
     misc_signal     3039..3059
                     /label=CAP_binding_site
                     /note="CAP binding site"
     -35_signal      3074..3079
     -10_signal      3098..3103
     primer_bind     3134..3160
                     /label=phiS3
     misc_feature    3148..3171
                     /gene="LacZ"
                     /note="Soltes 2006"
                     /label=LacZ
     misc_binding    3167..3172
                     /db_xref="REBASE:XbaI"
     CDS             3188..3250
                     /codon_start=1
                     /translation="MKYLLPTAAAGLLLLAAQPA*"
                     /label=PelB
                     /note="PelB leader peptide"
                     /gene="PelB"
     primer_bind     3188..3209
                     /label=phiS2
     misc_binding    3209..3216
                     /db_xref="REBASE:NotI"
     misc_binding    3235..3247
                     /db_xref="REBASE:SfiI"
     misc_binding    3236..3246
                     /db_xref="REBASE:BglI"
     misc_binding    3250..3255
                     /db_xref="REBASE:SpeI"
     misc_binding    3256..3268
                     /db_xref="REBASE:SfiI"
     misc_binding    3257..3267
                     /db_xref="REBASE:BglI"
     misc_binding    3297..3304
                     /db_xref="REBASE:AbsI"
     misc_binding    3298..3303
                     /db_xref="REBASE:XhoI"
     misc_binding    3304..3311
                     /db_xref="REBASE:AscI"
     misc_binding    3305..3310
                     /db_xref="REBASE:BssHII"
     primer_bind     complement(3343..3370)
                     /label=psiR2
     primer_bind     complement(3381..3404)
                     /label=psiR3
     misc_binding    3900..3905
                     /db_xref="REBASE:BamHI"
     misc_binding    4536..4541
                     /db_xref="REBASE:HindIII"
     rep_origin      4661..4967
                     /gene="orif1"
                     /label=oriF1
                     /note="origin of replication for phage f1, negative strand"
     CDS             3256..3312
                     /codon_start=1
                     /translation="GPGGQGGGSEGGGSLEGAP"
                     /gene
                     /label=Linker
                     /note
     CDS             3313..4533
                     /codon_start=1
                     /translation="AETVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVV
                     VCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTY
                     INPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTD
                     PVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAG
                     GGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENAD
                     ENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDN
                     SPLMNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMY
                     VFSTFANILRNKES*"
                     /gene="Gene III"
                     /label=g3p
                     /note
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc gcttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgacccg acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg
     1861 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
     1921 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
     1981 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
     2041 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
     2101 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
     2161 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
     2221 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
     2281 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
     2341 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
     2401 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
     2461 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
     2521 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
     2581 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
     2641 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggacat ctcggtagtg
     2701 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
     2761 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
     2821 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     2881 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     2941 caggtttccc gactggaaag cgggcagtga gcggtacccg ataaaagcgg cttcctgaca
     3001 ggaggccgtt ttgttttgca gcccacctca acgcaattaa tgtgagttag ctcactcatt
     3061 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     3121 gataacaatt tcacacagga aacagctatg accatgatta cgaatttcta gataacgagg
     3181 gcaaatcatg aaatacctat tgcctacggc ggccgctgga ttgttattac tcgcggccca
     3241 gccggcctaa ctagtggccc gggaggccaa ggcggtggtt ctgagggtgg tggctccctc
     3301 gagggcgcgc cagccgaaac tgttgaaagt tgtttagcaa aacctcatac agaaaattca
     3361 tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggctgt
     3421 ctgtggaatg ctacaggcgt tgtggtttgt actggtgacg aaactcagtg ttacggtaca
     3481 tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
     3541 tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
     3601 attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
     3661 aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
     3721 cagaataata ggttccgaaa taggcagggt gcattaactg tttatacggg cactgttact
     3781 caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
     3841 tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag
     3901 gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
     3961 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggcgg ctctgagggt
     4021 ggcggttctg agggtggcgg ctctgagggt ggcggttccg gtggcggctc cggttccggt
     4081 gattttgatt atgaaaaaat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
     4141 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
     4201 gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact
     4261 ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct
     4321 ttaatgaata atttccgtca atatttacct tctttgcctc agtcggttga atgtcgccct
     4381 tatgtctttg gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta
     4441 ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttcgacg
     4501 tttgctaaca tactgcgtaa taaggagtct taatcaagct ttaatatttt gttaaaattc
     4561 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     4621 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     4681 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     4741 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     4801 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     4861 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     4921 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     4981 gcgtcaggtg
//

