LOCUS       fADL-2blue              7861 bp    DNA     circular
DEFINITION  Filamentous phage cloning vector fADL-2blue, complete sequence.
ACCESSION   
VERSION     .4
KEYWORDS    .
COMMENT     Filamentous phage cloning vector constructed by splicing the
            ampicillin resistance determinant into the minus-strand
            origin of bacteriphage fd. Transduce ampicillin resistance into
            infected host cell. Double-stranded RF copy number is about 60
            copies in stationary-phase cells.  Yield of physical particles is
            about 5e11 virions/ml.  Make  very small plaques and large colonies.
             Display on gene III protein up to 100% with limited polyphage
            production.
            A copy of LacZ-Alpha peptide under the control of the lac promoter
            enables blue colonies in the presence of IPTG and X-gal on MC1061
            strain and derivatives.
            FT color
            FT join(6054..6104,6113..6118,6127..6146)
            FT /number="10"
            FT color
            FT join(5777..5797,5812..5838,5839..5840,5841,6105..6112,6119..6126,
            FT 7259..7273)
            FT /number="1"
            FT color
            FT join(5756..5776,5848..5868)
            FT /number="19"
            FT color
            FT join(5671..5755,5798..5811,5842..5847,5869..5885)
            FT /number="27"
            FT color
            FT 7239..7258
            FT /number="2"
            FT color
            FT 6147..7132
            FT /number="9"
            FT translated
            FT 1690
            FT /frame="1"
            FT translated
            FT 5886..6065
            FT /frame="3"
FEATURES             Location/Qualifiers
     CDS             join(7460..7861,1..831)
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein pII"
                     /protein_id="AAF75600.1"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSA
                     RTVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGH
                     NVFGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVI
                     DHLRNVSNGQTKSTRSQNWESTVTWNKTSRHRTLVAYLKHVELQHQIQQLSSKPSAKM
                     TSYQKEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNRQG
                     KDLIFDLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYF
                     GFYRRLVNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINV
                     DFSSQRPDWYNEPVLKIA*"
                     /note="truncated"
     CDS             496..831
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein pX"
                     /protein_id="AAF75601.1"
                     /translation="MNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRLVNE
                     GYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRPDW
                     YNEPVLKIA"
     CDS             843..1106
                     /codon_start=1
                     /transl_table=11
                     /product="cytosolic single-stranded DNA binding protein pV"
                     /protein_id="AAF75603.1"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVL
                     VKITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     CDS             1108..1209
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein pVII"
                     /protein_id="AAF75604.1"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     CDS             1206..1304
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein pIX"
                     /protein_id="AAF75605.1"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     CDS             1301..1522
                     /codon_start=1
                     /transl_table=11
                     /product="major coat protein pVIII precursor"
                     /protein_id="AAF75598.1"
                     /translation="MKKSLVLKASVAVATLVPMLSFAAEGDDPAKAAF"
     sig_peptide     1301..1369
     misc_binding    1565..1570
                     /db_xref="REBASE:SalI"
     CDS             1586..1651
                     /codon_start=1
                     /translation="MKYLLPTAAAGLLLLAAQPAM"
                     /label=PelB
     misc_binding    1607..1614
                     /db_xref="REBASE:NotI"
     misc_binding    1633..1645
                     /db_xref="REBASE:SfiI"
     misc_binding    1634..1644
                     /db_xref="REBASE:BglI"
     misc_binding    1651..1656
                     /db_xref="REBASE:MluI"
     misc_binding    1657..1662
                     /db_xref="REBASE:SpeI"
     CDS             1663..1689
                     /codon_start=1
                     /translation="GPGGLRGGS"
                     /gene
                     /label=Linker
                     /note
     misc_binding    1663..1675
                     /db_xref="REBASE:SfiI"
     misc_binding    1664..1674
                     /db_xref="REBASE:BglI"
     CDS             1690..2910
                     /codon_start=1
                     /translation="AETVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVV
                     VCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTY
                     INPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTD
                     PVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAG
                     GGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENAD
                     ENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDN
                     SPLMNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMY
                     VFSTFANILRNKES*"
                     /label=g3p
     primer_bind     complement(1720..1747)
                     /label=psiR2
     primer_bind     complement(1758..1781)
                     /label=psiR3
     misc_binding    2277..2282
                     /db_xref="REBASE:BamHI"
     CDS             2913..3251
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein pVI"
                     /protein_id="AAF75606.1"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFL
                     ALIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQ
                     KIVSYLDWDK*"
     CDS             3254..4300
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein pI"
                     /protein_id="AAF75599.1"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLP
                     QVGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDK
                     ERQPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTL
                     YSLVTGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGV
                     YSYLTPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPE
                     VKKVVSQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSITYIDLCTV
                     SIKKGNSNEIVKCN*"
     CDS             4278..5558
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein IV"
                     /protein_id="AAF75602.1"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQTGE
                     SVIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNSQDYI
                     DELPSSDIQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKS
                     SNVLSVDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFA
                     AGSQRGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRIL
                     TLSGQKGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIV
                     LDITSKADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFL
                     SKIPLIGLLFSSRSDSNEESTLYVLVKATIVRAL*"
     misc_feature    5557..5634
                     /note="morphogenetic protein signal sequence; promotes
                     packaging of circular single plus strands into progeny
                     virions; other site"
                     /citation=[3]
     misc_structure  5557..5634
                     /label=Packaging_Signal
                     /color="20"
     misc_binding    5756..5776
                     /label=operator_O3
     misc_binding    5777..5797
                     /label=CAP_binding
     -35_signal      5812..5817
     -10_signal      5836..5841
     misc_feature    5839..5840
                     /label=UV5
     misc_binding    5848..5868
                     /label=operator_O1
     misc_binding    5990..5995
                     /db_xref="REBASE:PvuII"
     CDS             complement(6147..7007)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
     promoter        complement(7049..7077)
                     /label=AmpR_Promoter
     misc_structure  7146..7204
                     /gene
                     /label=Loop_C
                     /note
                     /color="10"
     rep_origin      7219..7358
                     /note="plus strand origin"
                     /citation=[3]
     misc_structure  7219..7247
                     /label=Loop_D
                     /color="5"
     misc_structure  7239..7273
                     /label=loop_E
                     /color="20"
     primer_bind     1486..1510
                     /gene
                     /label=phi8S3
                     /note
     primer_bind     1586..1607
                     /gene
                     /label=phiS2
                     /note
ORIGIN
        1 aacgctacta ccattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
       61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
      121 cgttcgcaga attgggaatc aactgttaca tggaataaaa cttccagaca ccgtacttta
      181 gttgcatatt taaaacatgt tgaactacag caccagattc agcaattaag ctctaagcca
      241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactgtctaa tcctgacctg
      301 ttggaatttg cttccggtct ggttcgcttt gaggctcgaa ttaaaacgcg atatttgaag
      361 tctttcgggc ttcctcttaa tctttttgat gcaattcgct ttgcttctga ctataataga
      421 cagggtaaag acctgatttt tgatttatgg tcattctctt tttctgaact gtttaaagca
      481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
      541 aaacatttta caattacccc ctctggcaaa acttcctttg caaaagcctc tcgctatttt
      601 ggtttctatc gtcgtctggt taatgagggt tatgatagtg ttgctcttac catgcctcgt
      661 aattcctttt ggcgttatgt atctgcatta gttgagtgtg gtattcctaa atctcaattg
      721 atgaatcttt ccacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
      781 tcctcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
      841 aaatgattaa agttgaaatt aaaccatctc aagcgcaatt tactacccgt tctggtgttt
      901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
      961 aatatccggt gcttgtcaag attactctcg acgaaggtca gccagcgtat gcgcctggtc
     1021 tgtacaccgt gcatctgtcc tcgttcaaag ttggtcagtt cggttctctt atgattgacc
     1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     1201 caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct
     1321 caaagcctcc gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     1561 ttttgtcgac taacgagggc aaatcatgaa atacctattg cctacggcgg ccgctggatt
     1621 gttattactc gcggcccagc cggccatgta acgcgtacta gtggcccggg aggcctgcgt
     1681 ggtggttcag ccgaaactgt tgaaagttgt ttagcaaaac ctcatacaga aaattcattt
     1741 actaacgtct ggaaagacga caaaacttta gatcgttacg ctaactatga gggctgtctg
     1801 tggaatgcta caggcgttgt ggtttgtact ggtgacgaaa ctcagtgtta cggtacatgg
     1861 gttcctattg ggcttgctat ccctgaaaat gagggtggtg gctctgaggg tggcggttct
     1921 gagggtggcg gttctgaggg tggcggtact aaacctcctg agtacggtga tacacctatt
     1981 ccgggctata cttatatcaa ccctctcgac ggcacttatc cgcctggtac tgagcaaaac
     2041 cccgctaatc ctaatccttc tcttgaggag tctcagcctc ttaatacttt catgtttcag
     2101 aataataggt tccgaaatag gcagggtgca ttaactgttt atacgggcac tgttactcaa
     2161 ggcactgacc ccgttaaaac ttattaccag tacactcctg tatcatcaaa agccatgtat
     2221 gacgcttact ggaacggtaa attcagagac tgcgctttcc attctggctt taatgaggat
     2281 ccattcgttt gtgaatatca aggccaatcg tctgacctgc ctcaacctcc tgtcaatgct
     2341 ggcggcggct ctggtggtgg ttctggtggc ggctctgagg gtggcggctc tgagggtggc
     2401 ggttctgagg gtggcggctc tgagggtggc ggttccggtg gcggctccgg ttccggtgat
     2461 tttgattatg aaaaaatggc aaacgctaat aagggggcta tgaccgaaaa tgccgatgaa
     2521 aacgcgctac agtctgacgc taaaggcaaa cttgattctg tcgctactga ttacggtgct
     2581 gctatcgatg gtttcattgg tgacgtttcc ggccttgcta atggtaatgg tgctactggt
     2641 gattttgctg gctctaattc ccaaatggct caagtcggtg acggtgataa ttcaccttta
     2701 atgaataatt tccgtcaata tttaccttct ttgcctcagt cggttgaatg tcgcccttat
     2761 gtctttggcg ctggtaaacc atatgaattt tctattgatt gtgacaaaat aaacttattc
     2821 cgtggtgtct ttgcgtttct tttatatgtt gccaccttta tgtatgtatt ttcgacgttt
     2881 gctaacatac tgcgtaataa ggagtcttaa tcatgccagt tcttttgggt attccgttat
     2941 tattgcgttt cctcggtttc cttctggtaa ctttgttcgg ctatctgctt actttcctta
     3001 aaaagggctt cggtaagata gctattgcta tttcattgtt tcttgctctt attattgggc
     3061 ttaactcaat tcttgtgggt tatctctctg atattagcgc acaattaccc tctgattttg
     3121 ttcagggcgt tcagttaatt ctcccgtcta atgcgcttcc ctgtttttat gttattctct
     3181 ctgtaaaggc tgctattttc atttttgacg ttaaacaaaa aatcgtttct tatttggatt
     3241 gggataaata aatatggctg tttattttgt aactggcaaa ttaggctctg gaaagacgct
     3301 cgttagcgtt ggtaagattc aggataaaat tgtagctggg tgcaaaatag caactaatct
     3361 tgatttaagg cttcaaaacc tcccgcaagt cgggaggttc gctaaaacgc ctcgcgttct
     3421 tagaataccg gataagcctt ctatttctga tttgcttgct attggtcgtg gtaatgattc
     3481 ctacgacgaa aataaaaacg gtttgcttgt tcttgatgaa tgcggtactt ggtttaatac
     3541 ccgttcatgg aatgacaagg aaagacagcc gattattgat tggtttcttc atgctcgtaa
     3601 attgggatgg gatattattt ttcttgttca ggatttatct attgttgata aacaggcgcg
     3661 ttctgcatta gctgaacacg ttgtttattg tcgccgtctg gacagaatta ctttaccctt
     3721 tgtcggcact ttatattctc ttgttactgg ctcaaaaatg cctctgccta aattacatgt
     3781 tggtgttgtt aaatatggtg attctcaatt aagccctact gttgagcgtt ggctttatac
     3841 tggtaagaat ttatataacg catatgacac taaacaggct ttttccagta attatgattc
     3901 aggtgtttat tcatatttaa ccccttattt atcacacggt cggtatttca aaccattaaa
     3961 tttaggtcag aagatgaaat taactaaaat atatttgaaa aagttttctc gcgttctttg
     4021 tcttgcgata ggatttgcat cagcatttac atatagttat ataacccaac ctaagccgga
     4081 ggttaaaaag gtagtctctc agacctatga ttttgataaa ttcactattg actcttctca
     4141 gcgtcttaat ctaagctatc gctatgtttt caaggattct aagggaaaat taattaatag
     4201 cgacgattta cagaagcaag gttattccat cacatatatt gatttatgta ctgtttcaat
     4261 taaaaaaggt aattcaaatg aaattgttaa atgtaattaa ttttgttttc ttgatgtttg
     4321 tttcatcatc ttcttttgct caagtaattg aaatgaataa ttcgcctctg cgcgatttcg
     4381 tgacttggta ttcaaagcaa acaggtgaat ctgttattgt ctcacctgat gttaaaggta
     4441 cagtgactgt atattcctct gacgttaagc ctgaaaattt acgcaatttc tttatctctg
     4501 ttttacgtgc taataatttt gatatggttg gctctaatcc ttccataatt cagaaatata
     4561 acccaaatag tcaggattat attgatgaat tgccatcatc tgatattcag gaatatgatg
     4621 ataattccgc tccttctggt ggtttctttg ttccgcaaaa tgataatgtt actcaaacat
     4681 ttaaaattaa taacgttcgc gcaaaggatt taataagggt tgtagaattg tttgttaaat
     4741 ctaatacatc taaatcctca aatgtattat ctgttgatgg ttctaactta ttagtagtta
     4801 gcgcccctaa agatatttta gataaccttc cgcaatttct ttctactgtt gatttgccaa
     4861 ctgaccagat attgattgaa ggattaattt tcgaggttca gcaaggtgat gctttagatt
     4921 tttcctttgc tgctggctct cagcgcggca ctgttgctgg tggtgttaat actgaccgtc
     4981 taacctctgt tttatcttct gcgggtggtt cgttcggtat ttttaacggc gatgttttag
     5041 ggctatcagt tcgcgcatta aagactaata gccattcaaa aatattgtct gtgcctcgta
     5101 ttcttacgct ttcaggtcag aagggttcta tttctgttgg ccagaatgtc ccttttatta
     5161 ctggtcgtgt aactggtgaa tctgccaatg taaataatcc atttcagaca attgagcgtc
     5221 aaaatgttgg tatttctatg agtgtttttc ccgttgcaat ggctggcggt aatattgttt
     5281 tagatataac cagtaaggcc gatagtttga gttcttctac tcaggcaagt gatgttatta
     5341 ctaatcaaag aagtattgcg acaacggtta atttgcgtga tggtcagact cttttgctcg
     5401 gtggcctcac tgattacaaa aacacttctc aagattctgg tgtgccgttc ctgtctaaaa
     5461 tccctttaat cggcctcctg tttagctccc gttctgattc taacgaggaa agcacgttgt
     5521 acgtgctcgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5581 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5641 gctttcttcc cttcctttct cgccacgttc gcgcccaata cgcaaaccgc ctctccccgc
     5701 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     5761 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     5821 tatgcttccg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     5881 cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5941 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     6001 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aattaaaata
     6061 gataggtttt atttgaagct aaatcttctt tatcgtaaaa aatgccctct tgggttatca
     6121 agagggtcat tatatttcgc ggaatattac caatgcttaa tcagtgaggc acctatctca
     6181 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6241 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga tccacgctca
     6301 ccggctccag atttatcagc aataaaccag ccagccggaa gcgccgagcg cagaagtggt
     6361 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6421 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6481 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6541 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6601 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6661 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6721 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6781 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     6841 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     6901 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     6961 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7021 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7081 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctcga
     7141 cctccaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     7201 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7261 aacaacactc acaactaacc cggcctattc ttttgattta taaggatttt tgtcattttc
     7321 tgcttactgg ttaaaaaata agctgattta acaaatattt aacgcgaaat ttaacaaaac
     7381 attaacgttt acaatttaaa tatttgctta tacaatcatc ctgtttttgg ggcttttctg
     7441 attatcaatc ggggtacata tgattgacat gctagtttta cgattaccgt tcatcgattc
     7501 tcttgtttgc tccagacttt caggtaatga cctgatagcc tttgtagacc tctcaaaaat
     7561 agctaccctc tccggcatga atttatcagc tagaacggtt gaatatcata ttgacggtga
     7621 tttgactgtc tccggccttt ctcacccgtt tgaatctttg cctactcatt actccggcat
     7681 tgcatttaaa atatatgagg gttctaaaaa tttttatccc tgcgttgaaa ttaaggcttc
     7741 accagcaaaa gtattacagg gtcataatgt ttttggtaca accgatttag ctttatgctc
     7801 tgaggcttta ttgcttaatt ttgctaactc tctgccttgc ttgtacgatt tattggatgt
     7861 t
//
