LOCUS       CM13d3                  7391 bp ss-DNA     circular     27-APR-2020
DEFINITION  CM13d3 pIII-Defective Helper Phage.
ACCESSION   
VERSION     .2
KEYWORDS    .
COMMENT     
FEATURES             Location/Qualifiers
     gene            join(6990..7391,1..831)
                     /gene="II"
                     /locus_tag="M13p01"
                     /db_xref="GeneID:927328"
     CDS             join(6990..7391,1..831)
                     /gene="II"
                     /locus_tag="M13p01"
                     /note="The pII protein binds to the intergenic region in
                     double-stranded, supercoiled DNA (replicative form) and
                     nicks the positive strand.  The resulting free 3' OH
                     terminus serves as a primer for new strand synthesis using
                     host factors (Rep helicase, DNA polymerase III). After a
                     full round of replication, pII circularizes the displaced
                     DNA strand. Newly synthesized (+) single stranded DNA is
                     either converted to RF DNA or packaged as genomes,
                     depending on the concentrations of ssDNA binding protein pV
                     and a separately expressed fragment of pII termed pX.
                     Functions: DNA replication initiation"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="NP_510885.1"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSA
                     RTVEYHVDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGH
                     NVFGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVI
                     DHLRNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKM
                     TSYQKEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQG
                     KDLIFDLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYF
                     GFYRRLVNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINV
                     DFSSQRPDWYNEPVLKIA*"
                     /db_xref="GI:17426218"
                     /db_xref="GeneID:927328"
     gene            496..831
                     /gene="X"
                     /locus_tag="M13p02"
                     /db_xref="GeneID:927329"
     CDS             496..831
                     /gene="X"
                     /locus_tag="M13p02"
                     /note="Protein pX is synthesized from an in-frame internal
                     initiation codon in gene II and is identical to the
                     C-terminal third of pII.  Required for the accumulation of
                     (+) single stranded genomic DNA."
                     /codon_start=1
                     /transl_table=11
                     /product="gene X product"
                     /protein_id="NP_510886.1"
                     /translation="MNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRLVNE
                     GYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRPDW
                     YNEPVLKIA"
                     /db_xref="GI:17426219"
                     /db_xref="GeneID:927329"
     gene            843..1106
                     /gene="V"
                     /locus_tag="M13p03"
                     /db_xref="GeneID:927330"
     CDS             843..1106
                     /gene="V"
                     /locus_tag="M13p03"
                     /codon_start=1
                     /transl_table=11
                     /product="helix destabilising protein"
                     /protein_id="NP_510887.1"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVL
                     VKITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
                     /db_xref="GI:17426220"
                     /db_xref="GeneID:927330"
     gene            1108..1209
                     /gene="VII"
                     /locus_tag="M13p04"
                     /db_xref="GeneID:927331"
     CDS             1108..1209
                     /gene="VII"
                     /locus_tag="M13p04"
                     /note="pVII minor coat proteins of E. coli filamentous
                     phages are located at the tip of the virion that first
                     emerges from the host cell."
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein"
                     /protein_id="NP_510888.1"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
                     /db_xref="GI:17426221"
                     /db_xref="GeneID:927331"
     gene            1206..1304
                     /gene="IX"
                     /locus_tag="M13p05"
                     /db_xref="GeneID:927332"
     CDS             1206..1304
                     /gene="IX"
                     /locus_tag="M13p05"
                     /note="pVII minor coat proteins of E. coli filamentous
                     phages are located at the tip of the virion that first
                     emerges from the host cell."
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein"
                     /protein_id="NP_510889.1"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
                     /db_xref="GI:17426222"
                     /db_xref="GeneID:927332"
     gene            1301..1522
                     /gene="VIII"
                     /locus_tag="M13p06"
                     /db_xref="GeneID:927333"
     CDS             1301..1522
                     /gene="VIII"
                     /locus_tag="M13p06"
                     /note="Several thousand copies of the major coat protein
                     form the tube containing the ssDNA genome to form the
                     elongated body of filamentous phages."
                     /codon_start=1
                     /transl_table=11
                     /product="major coat protein"
                     /protein_id="NP_510890.1"
                     /translation="MKKSLALKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEY
                     IGYAWAMVVVIVGATIGIKLFKKFTSKAS*"
                     /db_xref="GI:17426223"
                     /db_xref="GeneID:927333"
     gene            1579..1917
                     /gene="VI"
                     /locus_tag="M13p08"
                     /db_xref="GeneID:927335"
     CDS             1579..1917
                     /gene="VI"
                     /locus_tag="M13p08"
                     /note="The pVI proteins of Ff phages are minor coat
                     proteins that are located at the end of the virion.  They
                     are involved in termination of assembly. In the absence of
                     pVI, phage particles remain associated with the host
                     membrane."
                     /codon_start=1
                     /transl_table=11
                     /product="minor coat protein"
                     /protein_id="NP_510892.1"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFL
                     ALIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQ
                     KIVSYLDWDK*"
                     /db_xref="GI:17426225"
                     /db_xref="GeneID:927335"
     gene            1919..2965
                     /gene="I"
                     /locus_tag="M13p09"
                     /db_xref="GeneID:927336"
     CDS             1919..2965
                     /gene="I"
                     /locus_tag="M13p09"
                     /note="The pI protein of filamentous phages is involved in
                     morphogenesis and may hydrolyze ATP. It is an inner
                     membrane protein and interacts with the outer membrane
                     protein pIV.  The N-termini of members of this cluster
                     share homology to the zonular occludens toxin (Zot) encoded
                     by phage CTXphi. However, the toxin activity of Zot was
                     mapped to the C terminus, which is divergent in the
                     proteins in this cluster. pXI is the product of
                     translational reinitiationin gene I and interacts with pI."
                     /codon_start=1
                     /transl_table=11
                     /product="assembly protein"
                     /protein_id="NP_510893.1"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLP
                     QVGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDK
                     ERQPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTL
                     YSLITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGV
                     YSYLTPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPE
                     VKKVVSQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTV
                     SIKKGNSNEIVKCN*"
                     /db_xref="GI:17426226"
                     /db_xref="GeneID:927336"
     gene            2943..4223
                     /gene="IV"
                     /locus_tag="M13p10"
                     /db_xref="GeneID:927337"
     CDS             2943..4223
                     /gene="IV"
                     /locus_tag="M13p10"
                     /note="pIV proteins of filamentous phages are multimeric
                     proteins that form a channel in the outer membrane through
                     which the phage particles exit the host cell. Function:
                     morphogenesis."
                     /codon_start=1
                     /transl_table=11
                     /product="phage assembly protein"
                     /protein_id="NP_510894.1"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGE
                     SVIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYI
                     DELPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKS
                     SNVLSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFA
                     AGSQRGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRIL
                     TLSGQKGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIV
                     LDITSKADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFL
                     SKIPLIGLLFSSRSDSNEESTLYVLVKATIVRAL*"
                     /db_xref="GI:17426227"
                     /db_xref="GeneID:927337"
     rep_origin      4211..4553
                     /note="M13 origin of replication (- +) (fragment)"
     rep_origin      complement(4769..5314)
                     /note="p15A origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
     gene            complement(5863..6678)
                     /gene="aph(3')-Ia"
     CDS             complement(5863..6678)
                     /gene="aph(3')-Ia"
                     /note="kanR (confers resistance to kanamycin)"
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase from Tn903"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     misc_binding    6124..6129
                     /db_xref="REBASE:HindIII"
     rep_origin      6815..6852
                     /note="M13 origin of replication (- +) (fragment)"
     misc_difference 7140
                     /gene
                     /label=ir3B
                     /note="ir3B mutation A[8417]>G"
ORIGIN
        1 aacgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
       61 atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
      121 cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
      181 gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
      241 tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
      301 ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
      361 tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
      421 cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
      481 tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
      541 aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
      601 ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
      661 aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
      721 atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
      781 tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
      841 caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt
      901 ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg
      961 aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc
     1021 tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc
     1081 gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat
     1141 caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt
     1201 caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta
     1261 gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagccct
     1321 caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga
     1381 cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta
     1441 tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa
     1501 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt
     1561 tttttggaga ttttcaacat gccagttctt ttgggtattc cgttattatt gcgtttcctc
     1621 ggtttccttc tggtaacttt gttcggctat ctgcttactt ttcttaaaaa gggcttcggt
     1681 aagatagcta ttgctatttc attgtttctt gctcttatta ttgggcttaa ctcaattctt
     1741 gtgggttatc tctctgatat tagcgctcaa ttaccctctg actttgttca gggtgttcag
     1801 ttaattctcc cgtctaatgc gcttccctgt ttttatgtta ttctctctgt aaaggctgct
     1861 attttcattt ttgacgttaa acaaaaaatc gtttcttatt tggattggga taaataatat
     1921 ggctgtttat tttgtaactg gcaaattagg ctctggaaag acgctcgtta gcgttggtaa
     1981 gattcaggat aaaattgtag ctgggtgcaa aatagcaact aatcttgatt taaggcttca
     2041 aaacctcccg caagtcggga ggttcgctaa aacgcctcgc gttcttagaa taccggataa
     2101 gccttctata tctgatttgc ttgctattgg gcgcggtaat gattcctacg atgaaaataa
     2161 aaacggcttg cttgttctcg atgagtgcgg tacttggttt aatacccgtt cttggaatga
     2221 taaggaaaga cagccgatta ttgattggtt tctacatgct cgtaaattag gatgggatat
     2281 tatttttctt gttcaggact tatctattgt tgataaacag gcgcgttctg cattagctga
     2341 acatgttgtt tattgtcgtc gtctggacag aattacttta ccttttgtcg gtactttata
     2401 ttctcttatt actggctcga aaatgcctct gcctaaatta catgttggcg ttgttaaata
     2461 tggcgattct caattaagcc ctactgttga gcgttggctt tatactggta agaatttgta
     2521 taacgcatat gatactaaac aggctttttc tagtaattat gattccggtg tttattctta
     2581 tttaacgcct tatttatcac acggtcggta tttcaaacca ttaaatttag gtcagaagat
     2641 gaaattaact aaaatatatt tgaaaaagtt ttctcgcgtt ctttgtcttg cgattggatt
     2701 tgcatcagca tttacatata gttatataac ccaacctaag ccggaggtta aaaaggtagt
     2761 ctctcagacc tatgattttg ataaattcac tattgactct tctcagcgtc ttaatctaag
     2821 ctatcgctat gttttcaagg attctaaggg aaaattaatt aatagcgacg atttacagaa
     2881 gcaaggttat tcactcacat atattgattt atgtactgtt tccattaaaa aaggtaattc
     2941 aaatgaaatt gttaaatgta attaattttg ttttcttgat gtttgtttca tcatcttctt
     3001 ttgctcaggt aattgaaatg aataattcgc ctctgcgcga ttttgtaact tggtattcaa
     3061 agcaatcagg cgaatccgtt attgtttctc ccgatgtaaa aggtactgtt actgtatatt
     3121 catctgacgt taaacctgaa aatctacgca atttctttat ttctgtttta cgtgcaaata
     3181 attttgatat ggtaggttct aacccttcca ttattcagaa gtataatcca aacaatcagg
     3241 attatattga tgaattgcca tcatctgata atcaggaata tgatgataat tccgctcctt
     3301 ctggtggttt ctttgttccg caaaatgata atgttactca aacttttaaa attaataacg
     3361 ttcgggcaaa ggatttaata cgagttgtcg aattgtttgt aaagtctaat acttctaaat
     3421 cctcaaatgt attatctatt gacggctcta atctattagt tgttagtgct cctaaagata
     3481 ttttagataa ccttcctcaa ttcctttcaa ctgttgattt gccaactgac cagatattga
     3541 ttgagggttt gatatttgag gttcagcaag gtgatgcttt agatttttca tttgctgctg
     3601 gctctcagcg tggcactgtt gcaggcggtg ttaatactga ccgcctcacc tctgttttat
     3661 cttctgctgg tggttcgttc ggtattttta atggcgatgt tttagggcta tcagttcgcg
     3721 cattaaagac taatagccat tcaaaaatat tgtctgtgcc acgtattctt acgctttcag
     3781 gtcagaaggg ttctatctct gttggccaga atgtcccttt tattactggt cgtgtgactg
     3841 gtgaatctgc caatgtaaat aatccatttc agacgattga gcgtcaaaat gtaggtattt
     3901 ccatgagcgt ttttcctgtt gcaatggctg gcggtaatat tgttctggat attaccagca
     3961 aggccgatag tttgagttct tctactcagg caagtgatgt tattactaat caaagaagta
     4021 ttgctacaac ggttaatttg cgtgatggac agactctttt actcggtggc ctcactgatt
     4081 ataaaaacac ttctcaggat tctggcgtac cgttcctgtc taaaatccct ttaatcggcc
     4141 tcctgtttag ctcccgctct gattctaacg aggaaagcac gttatacgtg ctcgtcaaag
     4201 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4261 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4321 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4381 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     4441 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4501 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggacggatc
     4561 gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat gtgatacagg
     4621 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg
     4681 gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg
     4741 gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc
     4801 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
     4861 cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
     4921 tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag
     4981 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc
     5041 ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca
     5101 gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta
     5161 aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga
     5221 gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga
     5281 gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta aggggtctga
     5341 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5401 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5461 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5521 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5581 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5641 agatttatca gcaataaacc agccagccga ttcgagctcg cccggggatc gaccagttgg
     5701 tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct
     5761 gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccgccgtccc gtcaagtcag
     5821 cgtaatgctc tgccagtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag
     5881 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     5941 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     6001 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     6061 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     6121 caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     6181 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa
     6241 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     6301 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     6361 tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     6421 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     6481 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     6541 cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     6601 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgagc aagacgtttc
     6661 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
     6721 tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac
     6781 gtggctttcc cccccccccc ctgcaggtct cgggctattc ttttgattta taagggattt
     6841 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6901 ttaacaaaat attaacgttt acaatttaaa tatttgctta tacaatcttc ctgtttttgg
     6961 ggcttttctg attatcaacc ggggtacata tgattgacat gctagtttta cgattaccgt
     7021 tcatcgattc tcttgtttgc tccagactct caggcaatga cctgatagcc tttgtagacc
     7081 tctcaaaaat agctaccctc tccggcatga atttatcagc tagaacggtt gaatatcatg
     7141 ttgatggtga tttgactgtc tccggccttt ctcacccttt tgaatcttta cctacacatt
     7201 actcaggcat tgcatttaaa atatatgagg gttctaaaaa tttttatcct tgcgttgaaa
     7261 taaaggcttc tcccgcaaaa gtattacagg gtcataatgt ttttggtaca accgatttag
     7321 ctttatgctc tgaggcttta ttgcttaatt ttgctaattc tttgccttgc ctgtatgatt
     7381 tattggatgt t
//

